Construct: shRNA TRCN0000440210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004740.3-2004s21c1
DNA Barcode:
TTCTCTCCTGCAATTGGTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TIAF1 (9220)
Gene Description:
TGFB1-induced anti-apoptotic factor 1
Transcript:
RefSeq NM_004740.3 (CURRENT)
Match location:
Position 2004 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000440210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTCTCCTGCAATTGGTTTG
Hairpin Sequence:
5'-CCGG-TTCTCTCCTGCAATTGGTTTG-CTCGAG-CAAACCAATTGCAGGAGAGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTCTCCTGCAATTGGTTTGCTCGAGCAAACCAATTGCAGGAGAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTCTCCTGCAATTGGTTTGCTCGAGCAAACCAATTGCAGGAGAGAA-3'

Other clones with same target sequence:

(none)