Construct: shRNA TRCN0000440924

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178908.3-794s21c1
DNA Barcode:
AGTGAGAGGAACCTCAAATGC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam26e (103511)
Gene Description:
family with sequence similarity 26, member E
Transcript:
RefSeq NM_178908.3 (CURRENT)
Match location:
Position 794 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000440924)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGAGAGGAACCTCAAATGC
Hairpin Sequence:
5'-CCGG-AGTGAGAGGAACCTCAAATGC-CTCGAG-GCATTTGAGGTTCCTCTCACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGAGAGGAACCTCAAATGCCTCGAGGCATTTGAGGTTCCTCTCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGAGAGGAACCTCAAATGCCTCGAGGCATTTGAGGTTCCTCTCACT-3'

Other clones with same target sequence:

(none)