Construct: shRNA TRCN0000441583

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016897.3-512s21c1
DNA Barcode:
AGGAGCACTTTGGGCTAATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Timm23 (53600)
Gene Description:
translocase of inner mitochondrial membrane 23
Transcript:
RefSeq NM_016897.3 (CURRENT)
Match location:
Position 512 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000441583)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAGCACTTTGGGCTAATAC
Hairpin Sequence:
5'-CCGG-AGGAGCACTTTGGGCTAATAC-CTCGAG-GTATTAGCCCAAAGTGCTCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAGCACTTTGGGCTAATACCTCGAGGTATTAGCCCAAAGTGCTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAGCACTTTGGGCTAATACCTCGAGGTATTAGCCCAAAGTGCTCCT-3'

Other clones with same target sequence:

(none)