Construct: shRNA TRCN0000442301

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011295.6-129s21c1
DNA Barcode:
ACGTCAACACTGCTCTACAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rps12 (20042)
Gene Description:
ribosomal protein S12
Transcript:
RefSeq NM_011295.6 (CURRENT)
Match location:
Position 129 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000442301)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTCAACACTGCTCTACAAG
Hairpin Sequence:
5'-CCGG-ACGTCAACACTGCTCTACAAG-CTCGAG-CTTGTAGAGCAGTGTTGACGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTCAACACTGCTCTACAAGCTCGAGCTTGTAGAGCAGTGTTGACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTCAACACTGCTCTACAAGCTCGAGCTTGTAGAGCAGTGTTGACGT-3'

Other clones with same target sequence:

(none)