Construct: shRNA TRCN0000442472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029999.4-948s21c1
DNA Barcode:
GTGAGCAAATGAGCAAGAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lbh (77889)
Gene Description:
limb-bud and heart
Transcript:
RefSeq NM_029999.4 (CURRENT)
Match location:
Position 948 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000442472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAGCAAATGAGCAAGAAAG
Hairpin Sequence:
5'-CCGG-GTGAGCAAATGAGCAAGAAAG-CTCGAG-CTTTCTTGCTCATTTGCTCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAGCAAATGAGCAAGAAAGCTCGAGCTTTCTTGCTCATTTGCTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAGCAAATGAGCAAGAAAGCTCGAGCTTTCTTGCTCATTTGCTCAC-3'

Other clones with same target sequence:

(none)