Construct: shRNA TRCN0000442576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028243.3-1881s21c1
DNA Barcode:
AGTCTCTCTGGCCCATTGTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prcp (72461)
Gene Description:
prolylcarboxypeptidase (angiotensinase C)
Transcript:
RefSeq NM_028243.3 (CURRENT)
Match location:
Position 1881 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000442576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCTCTCTGGCCCATTGTAA
Hairpin Sequence:
5'-CCGG-AGTCTCTCTGGCCCATTGTAA-CTCGAG-TTACAATGGGCCAGAGAGACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCTCTCTGGCCCATTGTAACTCGAGTTACAATGGGCCAGAGAGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCTCTCTGGCCCATTGTAACTCGAGTTACAATGGGCCAGAGAGACT-3'

Other clones with same target sequence:

(none)