Construct: shRNA TRCN0000444394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152813.3-2314s21c1
DNA Barcode:
GGTTTGTGGTAGAAGATTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plcd3 (72469)
Gene Description:
phospholipase C, delta 3
Transcript:
RefSeq NM_152813.3 (CURRENT)
Match location:
Position 2314 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000444394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTTTGTGGTAGAAGATTATG
Hairpin Sequence:
5'-CCGG-GGTTTGTGGTAGAAGATTATG-CTCGAG-CATAATCTTCTACCACAAACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTTTGTGGTAGAAGATTATGCTCGAGCATAATCTTCTACCACAAACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTTTGTGGTAGAAGATTATGCTCGAGCATAATCTTCTACCACAAACC-3'

Other clones with same target sequence:

(none)