Construct: shRNA TRCN0000444727

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016914.2-779s21c1
DNA Barcode:
TGGCGAAGAGCTTCATGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prg3 (53856)
Gene Description:
proteoglycan 3
Transcript:
RefSeq NM_016914.2 (CURRENT)
Match location:
Position 779 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000444727)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGCGAAGAGCTTCATGCAAA
Hairpin Sequence:
5'-CCGG-TGGCGAAGAGCTTCATGCAAA-CTCGAG-TTTGCATGAAGCTCTTCGCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGCGAAGAGCTTCATGCAAACTCGAGTTTGCATGAAGCTCTTCGCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGCGAAGAGCTTCATGCAAACTCGAGTTTGCATGAAGCTCTTCGCCA-3'

Other clones with same target sequence:

(none)