Construct: shRNA TRCN0000445567

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019468.2-1186s21c1
DNA Barcode:
ACCAGAAGTGCAAGCGTAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
G6pd2 (14380)
Gene Description:
glucose-6-phosphate dehydrogenase 2
Transcript:
RefSeq NM_019468.2 (CURRENT)
Match location:
Position 1186 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000445567)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAGAAGTGCAAGCGTAATG
Hairpin Sequence:
5'-CCGG-ACCAGAAGTGCAAGCGTAATG-CTCGAG-CATTACGCTTGCACTTCTGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAGAAGTGCAAGCGTAATGCTCGAGCATTACGCTTGCACTTCTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAGAAGTGCAAGCGTAATGCTCGAGCATTACGCTTGCACTTCTGGT-3'

Other clones with same target sequence:

(none)