Construct: shRNA TRCN0000446051

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172872.3-1350s21c1
DNA Barcode:
GAGTGCACTGTAGCCCATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kank4 (242553)
Gene Description:
KN motif and ankyrin repeat domains 4
Transcript:
RefSeq NM_172872.3 (CURRENT)
Match location:
Position 1350 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000446051)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTGCACTGTAGCCCATTTA
Hairpin Sequence:
5'-CCGG-GAGTGCACTGTAGCCCATTTA-CTCGAG-TAAATGGGCTACAGTGCACTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTGCACTGTAGCCCATTTACTCGAGTAAATGGGCTACAGTGCACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTGCACTGTAGCCCATTTACTCGAGTAAATGGGCTACAGTGCACTC-3'

Other clones with same target sequence:

(none)