Construct: shRNA TRCN0000446407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199032.2-1392s21c1
DNA Barcode:
CATAGAGGAAGAGCGCGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cep135 (381644)
Gene Description:
centrosomal protein 135
Transcript:
RefSeq NM_199032.2 (CURRENT)
Match location:
Position 1392 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000446407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGAGGAAGAGCGCGATTA
Hairpin Sequence:
5'-CCGG-CATAGAGGAAGAGCGCGATTA-CTCGAG-TAATCGCGCTCTTCCTCTATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGAGGAAGAGCGCGATTACTCGAGTAATCGCGCTCTTCCTCTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGAGGAAGAGCGCGATTACTCGAGTAATCGCGCTCTTCCTCTATG-3'

Other clones with same target sequence:

(none)