Construct: shRNA TRCN0000446829

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133925.2-2807s21c1
DNA Barcode:
TACTAAAGTAGTCTCTGAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm28 (68272)
Gene Description:
RNA binding motif protein 28
Transcript:
RefSeq NM_133925.2 (CURRENT)
Match location:
Position 2807 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000446829)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTAAAGTAGTCTCTGAATG
Hairpin Sequence:
5'-CCGG-TACTAAAGTAGTCTCTGAATG-CTCGAG-CATTCAGAGACTACTTTAGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTAAAGTAGTCTCTGAATGCTCGAGCATTCAGAGACTACTTTAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTAAAGTAGTCTCTGAATGCTCGAGCATTCAGAGACTACTTTAGTA-3'

Other clones with same target sequence:

(none)