Construct: shRNA TRCN0000447159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009197.2-1217s21c1
DNA Barcode:
CCTACGTGCACCTGATGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc16a2 (20502)
Gene Description:
solute carrier family 16 (monocarboxylic acid transporters), member 2
Transcript:
RefSeq NM_009197.2 (CURRENT)
Match location:
Position 1217 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000447159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACGTGCACCTGATGAAAT
Hairpin Sequence:
5'-CCGG-CCTACGTGCACCTGATGAAAT-CTCGAG-ATTTCATCAGGTGCACGTAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACGTGCACCTGATGAAATCTCGAGATTTCATCAGGTGCACGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACGTGCACCTGATGAAATCTCGAGATTTCATCAGGTGCACGTAGG-3'

Other clones with same target sequence:

(none)