Construct: shRNA TRCN0000447531

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207506.2-982s21c1
DNA Barcode:
GCCAATTGTGAGGGCGCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAMD12 (401474)
Gene Description:
sterile alpha motif domain containing 12
Transcript:
RefSeq NM_207506.2 (CURRENT)
Match location:
Position 982 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000447531)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAATTGTGAGGGCGCTTTA
Hairpin Sequence:
5'-CCGG-GCCAATTGTGAGGGCGCTTTA-CTCGAG-TAAAGCGCCCTCACAATTGGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAATTGTGAGGGCGCTTTACTCGAGTAAAGCGCCCTCACAATTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAATTGTGAGGGCGCTTTACTCGAGTAAAGCGCCCTCACAATTGGC-3'

Other clones with same target sequence:

(none)