Construct: shRNA TRCN0000452199

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027157.3-728s21c1
DNA Barcode:
AGAACAATCTGTAATCCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Krtap1-5 (69664)
Gene Description:
keratin associated protein 1-5
Transcript:
RefSeq NM_027157.3 (CURRENT)
Match location:
Position 728 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000452199)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAACAATCTGTAATCCTATT
Hairpin Sequence:
5'-CCGG-AGAACAATCTGTAATCCTATT-CTCGAG-AATAGGATTACAGATTGTTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAACAATCTGTAATCCTATTCTCGAGAATAGGATTACAGATTGTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAACAATCTGTAATCCTATTCTCGAGAATAGGATTACAGATTGTTCT-3'

Other clones with same target sequence:

(none)