Construct: shRNA TRCN0000452199
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_027157.3-728s21c1
- DNA Barcode:
- AGAACAATCTGTAATCCTATT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Krtap1-5 (69664)
- Gene Description:
- keratin associated protein 1-5
- Transcript:
- RefSeq NM_027157.3 (CURRENT)
- Match location:
- Position 728 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000452199)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGAACAATCTGTAATCCTATT
- Hairpin Sequence:
-
5'-CCGG-AGAACAATCTGTAATCCTATT-CTCGAG-AATAGGATTACAGATTGTTCT-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGAACAATCTGTAATCCTATTCTCGAGAATAGGATTACAGATTGTTCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGAACAATCTGTAATCCTATTCTCGAGAATAGGATTACAGATTGTTCT-3'
Other clones with same target sequence:
(none)
