Construct: shRNA TRCN0000452375

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177155.4-722s21c1
DNA Barcode:
AGAATCATAGCTGCCATTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Klri2 (320407)
Gene Description:
killer cell lectin-like receptor family I member 2
Transcript:
RefSeq NM_177155.4 (CURRENT)
Match location:
Position 722 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000452375)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATCATAGCTGCCATTATC
Hairpin Sequence:
5'-CCGG-AGAATCATAGCTGCCATTATC-CTCGAG-GATAATGGCAGCTATGATTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATCATAGCTGCCATTATCCTCGAGGATAATGGCAGCTATGATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATCATAGCTGCCATTATCCTCGAGGATAATGGCAGCTATGATTCT-3'

Other clones with same target sequence:

(none)