Construct: shRNA TRCN0000453788

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021320.3-926s21c1
DNA Barcode:
TTGTCAAGAAGGGAGCTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ntn4 (57764)
Gene Description:
netrin 4
Transcript:
RefSeq NM_021320.3 (CURRENT)
Match location:
Position 926 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000453788)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTCAAGAAGGGAGCTATTT
Hairpin Sequence:
5'-CCGG-TTGTCAAGAAGGGAGCTATTT-CTCGAG-AAATAGCTCCCTTCTTGACAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTCAAGAAGGGAGCTATTTCTCGAGAAATAGCTCCCTTCTTGACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTCAAGAAGGGAGCTATTTCTCGAGAAATAGCTCCCTTCTTGACAA-3'

Other clones with same target sequence:

(none)