Construct: ORF TRCN0000465244
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009825.1_s317c1
- Derived from:
- ccsbBroadEn_11253
- DNA Barcode:
- TGGCACCTCTATTGTTGAAATTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NCOA4 (8031)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465244
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8031 | NCOA4 | nuclear receptor coactivator 4 | NM_001145262.1 | 91.6% | 83.6% | 1563_1698del;1842_1843insTGAAGGAATGGACAAGAGT |
| 2 | human | 8031 | NCOA4 | nuclear receptor coactivator 4 | NM_001145263.1 | 91.6% | 83.6% | 1563_1698del;1842_1843insTGAAGGAATGGACAAGAGT |
| 3 | human | 8031 | NCOA4 | nuclear receptor coactivator 4 | NM_005437.3 | 91.6% | 83.6% | 1563_1698del;1842_1843insTGAAGGAATGGACAAGAGT |
| 4 | human | 8031 | NCOA4 | nuclear receptor coactivator 4 | NM_001145261.1 | 89.3% | 81.5% | 1_48del;1611_1746del;1890_1891insTGAAGGAATGGACAAGAGT |
| 5 | human | 8031 | NCOA4 | nuclear receptor coactivator 4 | NM_001145260.1 | 88% | 79.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1791
- ORF length:
- 1725
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa taccttccaa gaccagagtg gcagctccag taatagagaa ccccttttga 121 ggtgtagtga tgcacggagg gacttggagc ttgctattgg tggagttctc cgggctgaac 181 agcaaattaa agataacttg cgagaggtca aagctcagat tcacagttgc ataagccgtc 241 acctggaatg tcttagaagc cgtgaggtat ggctgtatga acaggtggac cttatttatc 301 agcttaaaga ggagacactt caacagcagg ctcagcagct ctactcgtta ttgggccagt 361 tcaattgtct tactcatcaa ctggagtgta cccaaaacaa agatctagcc aatcaagtct 421 ctgtgtgcct ggagagactg ggcagtttga cccttaagcc tgaagattca actgtcctgc 481 tctttgaagc tgacacaatt actctgcgcc agaccatcac cacatttggg tctctcaaaa 541 ccattcaaat tcctgagcac ttgatggctc atgctagttc agcaaatatt gggcccttcc 601 tggagaagag aggctgtatc tccatgccag agcagaagtc agcatccggt attgtagctg 661 tccctttcag cgaatggctc cttggaagca aacctgccag tggttatcaa gctccttaca 721 tacccagcac cgacccccag gactggctta cccaaaagca gaccttggag aacagtcaga 781 cttcttccag agcctgcaat ttcttcaata atgtcggggg aaacctaaag ggcttagaaa 841 actggctcct caagagtgaa aaatcaagtt atcaaaagtg taacagccat tccactacta 901 gttctttctc cattgaaatg gaaaaggttg gagatcaaga gcttcctgat caagatgaga 961 tggacctatc agattggcta gtgactcccc aggaatccca taagctgcgg aagcctgaga 1021 atggcagtcg tgaaaccagt gagaagttta agctcttatt ccagtcctat aatgtgaatg 1081 attggcttgt caagactgac tcctgtacca actgtcaggg aaaccagccc aaaggtgtgg 1141 agattgaaaa cctgggcaat ctgaagtgcc tgaatgacca cttggaggcc aagaaaccat 1201 tgtccacccc cagcatggtt acagaggatt ggcttgtcca gaaccatcag gacccatgta 1261 aggtagagga ggtgtgcaga gccaatgagc cctgcacaag ctttgcagag tgtgtgtgtg 1321 atgagaattg tgagaaggag gctctgtata agtggcttct gaagaaagaa ggaaaggata 1381 aaaatgggat gcctgtggaa cccaaacctg agcctgagaa gcataaagat tccctgaata 1441 tgtggctctg tcctagaaaa gaagtaatag aacaaactaa agcaccaaag gcaatgactc 1501 cTTCTAGAAT TGCTGATTCC TTCCAAGTCA TAAAGAACAG CCCCTTGTCG GAGTGGCTTA 1561 TCAGGCCCCC ATACAAAGAA GGAAGTCCCA AGGAAGTGCC TGGTACTGAA GACAGAGCTG 1621 GCAAACAGAA GTATTACTTA ATTCACCTCT ACAGGAGGAA CATAACTTCC CCCCAGACCA 1681 TTATGGCCTC CCTGCAGTTT GTGATCTCTT TGCCTGTATG CAGCTTAAAG TTGATAAAGA 1741 GAAGTGGTTA TATCGAACTC CTCTACAGAT GTGAAGGAAT GGACAAGAGT TGCCCAACTT 1801 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1861 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1921 CTTGTGGAAA GGACGATGGC ACCTCTATTG TTGAAATTCC ACGCGTTAAG TCgacaatca 1981 acctctggat tacaaaattt gtgaaagatt