Vector: pLX_317
pLX_317 is not available for distribution as a Gateway Destination vector,
and users should request pLX_307 instead. This is because pLX_317, by definition, is pLX_307 plus a
barcode sequence, but this barcode is added in the same workflow as the ORF
insert. Thus, we only have individual clones of pLX_317 with both a
barcode and an ORF insert.
View vector indexVector Information:
- Vector Name:
- pLX_317
- Alias:
- pLX_TRC307WBC, pLX_TRC317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-Gateway-V5(c-term)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Cloning Site:
- Gateway - Destination
- Epitope Tag:
- V5
Sequence Data Files
For a free vector file viewer, try ApE or SnapGene Viewer.
| GenBank[?]File in GenBank flat-file format | FASTA | SnapGene[?]File in proprietary SnapGene .DNA format | Revision Notes | |
|---|---|---|---|---|
| 1 | GB | FASTA | DNA | (original version) |
Control Clones
| Clone ID | Sequenced %[?]Percentage of construct sequence that has been empirically verified through direct sequence data. | Perfect Match Genes[?]100% full-length nucleotide and amino acid (for coding transcripts) alignment to current RefSeq transcript. | Near Match Genes[?]At least 99% full-length nucleotide and amino acid (for coding transcripts) alignment to current RefSeq transcript. | Lower Match Genes[?]At least 50% full-length nucleotide and amino acid (for coding transcripts) alignment to current RefSeq transcript, with at least 90% sequence identity over aligned region. | Notes | Addgene[?]Link to this entity's page in the Addgene catalog, if available | |
|---|---|---|---|---|---|---|---|
| 1 | BRDN0000559468 | 30.3% | LUCIFERASE, Luciferase | n/a | |||
| 2 | BRDN0000559460 | 61.8% | BFP | GFP, eGFP | n/a | ||
| 3 | BRDN0000559463 | 72.2% | HcRed | n/a | |||
| 4 | BRDN0000559461 | 14.2% | LacZ | lacZ | n/a | ||
| 5 | BRDN0000559466 | 70% | eGFP | GFP | BFP | n/a |