Construct: ORF TRCN0000465261
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013749.1_s317c1
- Derived from:
- ccsbBroadEn_12189
- DNA Barcode:
- CCCGGCCCACGTAACTTCTTTGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC47A1 (55244)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465261
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55244 | SLC47A1 | solute carrier family 47 me... | NM_018242.3 | 50.4% | 50.3% | (many diffs) |
| 2 | mouse | 67473 | Slc47a1 | solute carrier family 47, m... | XM_011249188.2 | 35.8% | 36.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 936
- ORF length:
- 870
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agctcctgag gagcccgcgc cagtgcgcgg aggcccggag gccacccttg 121 aggtccgtgg gtcgcgctgc ttgcggctgt ccgccttccg agaagagctg cgggcgctct 181 tggtcctggc tggccccgcg ttcttggttc agctgatggt gttcctgatc agcttcataa 241 gctccgtgtt ctgtggccac ctgggcaagc tggagctgga tgcagtcacg ctggcaatcg 301 cggttatcaa tgtcactggt gtctcagtgg gattcggctt atcttctgcc tgtgacaccc 361 tcatctccca gacgtacggg agccagaacc tgaagcacgt gggcgtgatc ctgcagcgga 421 gtgcgctcgt cctgctcctc tgctgcttcc cctgctgggc gctctttctc aacacccagc 481 acatcctgct gctcttcagg caggacccag atgtgtccag gcttacccag acctatgtca 541 cgatcttcat tccagctctt cctgcaacct ttctttatat gttacaagtt aaatatttgc 601 tcaaccaggg aattgtactg ccccagatcg taactGGAGT TGCAGCCAAC CTTGTCAATG 661 CCCTCGCCAA CTATCTGTTT CTCCATCAAC TGCATCTTGG GGTGATAGGC TCTGCACTGG 721 CAAACTTGAT TTCCCAGTAC ACCCTGGCTC TACTCCTCTT TCTCTACATC CTCGGGAAAA 781 AACTGCATCA AGCTACATGG GGAGGCTGGT CCCTCGAGTG CCTGCAGGAC TGGGCCTCCT 841 TCCTCCGCCT GGCCATCCCC AGCATGCTCA TGCTGTGCAT GGAGTGGTGG GCCTATGAGG 901 TCGGGAGCTT CCTCAGTGGT CTGTATGAGG ATGGATGCCC AACTTTCTTG TACAAAGTGG 961 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1021 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1081 ACCCGGCCCA CGTAACTTCT TTGTGACGCG TTAAGTCgac aatcaacctc tggattacaa 1141 aatttgtgaa agatt