Construct: ORF TRCN0000465281
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005970.1_s317c1
- Derived from:
- BRDN0000394374
- DNA Barcode:
- GCCATTATGCCAACTTATAGTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HOMEZ (57594)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465281
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57594 | HOMEZ | homeobox and leucine zipper... | NM_020834.3 | 94.9% | 94.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1644
- ORF length:
- 1575
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctcctaat aaagaggcca gcgaaatcag tagttcacca gcggggctca 121 tctgcctccc tccaatctct gaggagctac agcttgtgtg gacgcaagca gcccagacca 181 gtgagctaga cagcaatgaa cacctgctca aaaccttcag ctactttccc tatcccagcc 241 tagcagacat tgcccttctc tgcctacgtt atgggttgca gatggagaaa gtcaagactt 301 ggtttatggc ccagcgcctc cgctgtggta ttagctggtc atctgaagaa atagaagaga 361 ctcgagcccg agtagtctac cgtcgggacc aactccattt caaatccctt ctctctttta 421 ctcatcatgc gggacggccc ccagaggagg tgcctcctcc tccagtgcca gctccagagc 481 aagttggtat tggaataggt cctccaactc ttagcaagcc tacccagacg aaaggattga 541 aggtagagcc tgaggagccc tctcagatgc caccactgcc acagagtcac cagaaattaa 601 aggagtccct gatgacacct ggcagtggag cattccccta ccaatcagat ttttggcaac 661 atcttcaaag cagtggcctc tcaaaggagc aggcaggcag gggtcccaac cagtcacatg 721 gcataggtac tgcttcctgg aaccactcca caaccgtccc ccagccacaa gctcgggata 781 aacccccacc aattgcatta attgccagta gttgtaagga ggagtcagca tctagtgtta 841 ctccctcttc ttcctctacc tcttcttctt tccaggtact ggctaatgga gctactgcca 901 cctctaaacc cctccagcca ctaggctgtg tcccacagtc agtgtcaccc agtgaacagg 961 cattaccccc acatctggaa ccagcctggc cccaagggct acggcataac tcagtaccag 1021 gtagagttgg ccccacagag tacctttccc cagatatagg aggccagcga aagaccaagc 1081 gcaaaaccaa agagcagctg gctatcctta aatccttttt tttacagtgc cagtgggcac 1141 ggcgtgagga ttaccaaaag ttagaacaga tcactggttt acctcggcct gagatcattc 1201 agtggtttgg tgacacacgc tatgccttga agcatgggca actaaagtgg tttcgggaca 1261 acgcagtacc tggtgcccct agtttccaag acccagcaat tcctacacca ccaccatcaa 1321 cccgctcctt gaacgaaagg gctgagacac CACCTCTGCC GATCCCTCCA CCCCCACCGG 1381 ATATACAACC CTTGGAGAGG TACTGGGCAG CCCACCAACA GCTACGGGAA ACTGATATCC 1441 CTCAATTGAG TCAGGCATCA AGGCTTAGCA CCCAGCAGGT GCTGGATTGG TTTGACTCTC 1501 GATTACCTCA GCCAGCTGAG GTGGTAGTTT GTCTAGATGA AGAAGAGGAA GAGGAGGAGG 1561 AAGAACTGCC AGAAGATGAT GAGGAAGAGG AGGAGGAGGA GGAGGAAGAT GATGATGATG 1621 ATGATGATGT GATCATACAA GACTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1681 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1741 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CCATTATGCC 1801 AACTTATAGT TTGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1861 att