Construct: ORF TRCN0000465415
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008323.1_s317c1
- Derived from:
- ccsbBroadEn_10750
- DNA Barcode:
- AGTCCGAGTGCTACAAAAACAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATF6B (1388)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465415
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1388 | ATF6B | activating transcription fa... | NM_004381.5 | 42.9% | 39.6% | (many diffs) |
| 2 | human | 1388 | ATF6B | activating transcription fa... | NM_001136153.2 | 42.5% | 39% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagctgatg ctgctcagcg agattgctga cccgacgcgt ttcttcaccg 121 acaacctgct tagcccggag gactggggtc tgcagaacag caccttgtat tctggcctag 181 atgaagtggc cgaggagcag acgcagctct tccgttgccc ggagcaggat gtcccgtttg 241 acggcagctc cctggacgtg gggatggatg tcagcccctc tgagccccca tgggaactcc 301 tgccgatctt cccagatctt caggtgaagt ctgagccatc ttccccctgc tcttcctcct 361 ccctcagctc cgagtcatcg cgtctctcca cagagccatc cagcgaggct cttggggtag 421 gggaggtgct ccatgtgaag acagagtcct tggcaccccc actgtgtctc ctgggagatg 481 acccaacatc ctcatttgaa accgtccaga tcaacgttat ccccacctct gatgattcct 541 cagatgtcca gaccaagata gaacctgtct ctccatgttc ttccgtcaac tctgaggcct 601 ccctgctctc ggccgactcc tccagccagg cttttatagg agaggaggtc ctggaagtga 661 agacagagtc cctgtcccct tcaggatgcc tcctgtggga tgtcccagcc ccctcacttG 721 GAGCTGTCCA GATCAGCATG GGCCCATCCC TTGATGGCTC CTCAGGCAAA GCCCTGCCCA 781 CCCGGAAGCC GCCACTGCAG CCCAAACCTG TAGTGCTAAC CACTGTCCCA ATGCCATCCA 841 GAGCTGTGTC TCCCAGCACC ACAGTCCTTC TGCAGTCCCT CGTCCAGCCA CCCCCAGGTA 901 CTGAAGAAGG AGAAAAGGGC CGGGCATGGT GGCTCACGCC GGTAATCCCA GCACTTTGGG 961 AGGCTGAGGC GGGCGAATCA CCTGAGGTCA GAAGTTTAAG ACCAGCCTGG CCAACGTGGT 1021 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGAAGTCC GAGTGCTACA AAAACAAATA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt