Construct: ORF TRCN0000465443
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008316.1_s317c1
- Derived from:
- ccsbBroadEn_12530
- DNA Barcode:
- GGGTGATCCCACGCAGCTGAACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SMIM2 (79024)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465443
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79024 | SMIM2 | small integral membrane pro... | NM_024058.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 321
- ORF length:
- 255
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgGCATGGA AGCTGGGGAG AGGATTGATG CCAGCCAGCT GCCTCACAGG GTGCTGGAAA 121 CACGTGGCCA TGCCATTAGC ATCCTGTTCG GCTTCTGGAC GAGTTTCATC TGTGACACCT 181 ACATAGTCCT CGCTTGGATC AGCAAGATAA AAGGCAGCCC TGATGTTAGT GCCTCCTCTG 241 ATGAGCCATA CGCCAGAATC CAGCAGAGCA GAAGGCAATG CCACGCAGAA GAGGACCAAA 301 GTCAGGTGCC AGAAGCAGGC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 361 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 421 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGGT GATCCCACGC 481 AGCTGAACCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt