Construct: ORF TRCN0000465468
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016916.1_s317c1
- Derived from:
- ccsbBroadEn_08376
- DNA Barcode:
- ATTCGTTTGTTTACACGGACATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCTD5 (54442)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465468
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54442 | KCTD5 | potassium channel tetrameri... | NM_018992.4 | 99.8% | 100% | 699G>A |
| 2 | mouse | 69259 | Kctd5 | potassium channel tetrameri... | NM_027008.2 | 91.3% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 768
- ORF length:
- 702
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggagaatcac tgcgagctcc tgtcgccggc ccggggcggc atcggggcgg 121 ggctgggggg cggcctgtgc cgccgctgca gcgctgggct cggcgccctg gcccagcgcc 181 ctggcagcgt gtccaagtgg gtccgactca acgtcggcgg cacctacttc ctcaccactc 241 ggcagaccct gtgccgggac ccgaaatcct tcctgtaccg cttatgccag gccgatcccg 301 acctggactc agacaaggat gaaacaggcg cctatttaat cgacagagac cccacctact 361 ttgggcctgt gctgaactac cTGAGACACG GCAAGCTGGT GATTAACAAA GACCTCGCGG 421 AGGAAGGAGT GTTGGAGGAA GCAGAATTTT ACAATATCAC CTCATTAATA AAACTTGTAA 481 AGGACAAAAT TAGAGAACGA GACAGCAAAA CATCGCAGGT GCCTGTGAAG CATGTGTACC 541 GTGTGCTGCA GTGCCAGGAG GAGGAGCTCA CGCAGATGGT GTCCACCATG TCCGACGGCT 601 GGAAGTTCGA GCAGTTGGTC AGCATCGGCT CCTCTTACAA CTATGGGAAC GAAGACCAAG 661 CCGAGTTCCT CTGTGTGGTG TCCAAGGAGC TGCACAACAC CCCGTACGGT ACGGCCAGCG 721 AGCCCAGCGA GAAGGCCAAG ATTTTGCAAG AACGAGGCTC AAGAATGTGC CCAACTTTCT 781 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 841 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 901 GTGGAAAGGA CGAATTCGTT TGTTTACACG GACATTGACG CGTTAAGTCg acaatcaacc 961 tctggattac aaaatttgtg aaagatt