Construct: ORF TRCN0000465489
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015892.1_s317c1
- Derived from:
- ccsbBroadEn_01343
- DNA Barcode:
- GTGGTAATTTTAACGGACCCTATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTPN9 (5780)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465489
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5780 | PTPN9 | protein tyrosine phosphatas... | NM_002833.4 | 100% | 100% | |
| 2 | mouse | 56294 | Ptpn9 | protein tyrosine phosphatas... | NM_019651.2 | 91.6% | 97.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1845
- ORF length:
- 1779
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gcccgcgacc gcgccccggc ccgacatggc gccggagctg accccggagg 121 aggagcaggc taccaagcag tttctcgaag agattaacaa gtggacagtt cagtacaatg 181 tttccccgct gtcttggaat gtggctgtca agttcctcat ggcaaggaag tttgatgtgc 241 tccgtgccat agaattgttc cactcctaca gagaaactcg aaggaaggaa ggcattgtaa 301 agctgaaacc tcatgaggaa cctcttcgtt ctgagatcct cagtggaaaa ttcaccatct 361 taaatgttcg ggacccaaca ggagcctcca ttgccctctt tactgccagg ttgcatcatc 421 cccacaagtc agtccaacat gtggtacttc aggctctgtt ttacttgcta gacagagctg 481 tggatagctt tgaaactcag aggaatggac tggtgtttat ctatgacatg tgtggttcta 541 attatgccaa ctttgagctg gatcttggca agaaagtcct aaacctgctg aagggagcat 601 ttccagctcg tttgaagaag gtgctgattg tgggggcacc catatggttc cgagtgccct 661 attccatcat cagtctcctc ctgaaggaca aagtccggga gaggattcaa atattaaaga 721 catctgaggt cacgcagcat ctgcccaggg agtgtcttcc agaaaacctg ggtgggtacg 781 tcaaaattga tctcgccact tggaatttcc agttcctacc ccaggtgaac ggccacccag 841 atcccttcga tgagatcatc ctgttctccc tccctcctgc cttagactgg gactcagtac 901 atgttccagg tccccatgct atgaccatcc aagagttggt ggactatgtt aatgccaggc 961 aaaagcaagg aatctatgag gaatatgaag acattcgtcg tgagaaccct gttggcactt 1021 tccactgttc catgtctcca ggaaacctag agaaaaaccg ttatggggat gtaccctgcc 1081 tggaccaaac tagagtgaag ctaacaaagc gaagtggcca tactcagaca gattacatca 1141 atgccagttt catggatggc tacaagcaga agaatgctta cattggcaca caaggtcctt 1201 tggagaatac ctatcgtgat ttctggctca tggtatggga gcaaaaagtc ttggtgattg 1261 tcatgaccac ccgctttgag gaaggcggca ggagaaagtg tggccagtac tggcctttag 1321 aaaaagactc tcggatccga tttggcttcc tcacagtgac caatctaggc gtggagaaca 1381 tgaatcatta taagaaaaca acgctagaaa ttcacaacac agaggaacgg cagaaacgcc 1441 aggtgaccca cttccagttc ttgagctggc cagactatgg tgtcccttcc TCAGCAGCTT 1501 CCCTCATTGA CTTCTTGAGA GTGGTCAGAA ACCAGCAGAG TCTGGCTGTG AGCAACATGG 1561 GAGCACGCTC CAAAGGGCAG TGCCCTGAGC CACCCATTGT GGTCCATTGC AGTGCAGGCA 1621 TTGGCAGGAC AGGTACCTTC TGCTCACTGG ACATCTGCCT GGCACAGCTG GAGGAGCTTG 1681 GCACCCTTAA TGTGTTCCAG ACGGTGTCAC GCATGAGGAC CCAGAGGGCC TTCAGCATCC 1741 AGACCCCTGA GCAGTACTAT TTTTGCTACA AGGCCATCCT GGAGTTCGCA GAGAAGGAGG 1801 GCATGGTATC CTCTGGCCAA AACCTGCTGG CCGTGGAGAG TCAGTACCCA ACTTTCTTGT 1861 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1921 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1981 GAAAGGACGA GTGGTAATTT TAACGGACCC TATAACGCGT TAAGTCgaca atcaacctct 2041 ggattacaaa atttgtgaaa gatt