Construct: ORF TRCN0000465519
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002696.1_s317c1
- Derived from:
- ccsbBroadEn_10367
- DNA Barcode:
- CATCAGTGCAGATACTCGCAGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FUNDC2P2 (388965)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465519
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 65991 | FUNDC2 | FUN14 domain containing 2 | NM_023934.4 | 75.6% | 73.5% | (many diffs) |
| 2 | human | 388965 | FUNDC2P2 | FUN14 domain containing 2 p... | NR_003663.1 | 29.8% | 1_137del;591_1519del | |
| 3 | mouse | 67391 | Fundc2 | FUN14 domain containing 2 | NM_026126.4 | 85.6% | 86% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcgtccagt caaggaaact ttgagggaga tattgagtca gtggaccttg 121 cagaatttgc taagcagcag ccgtggtggc gtaagctgtt tgggccagaa tctggactct 181 cagccgaaaa gtatagcgtg gcaacccacc tgttcattGG AGGTGTCACT GGATGGTGCA 241 CGGGTTTTAT ATTCCAGAAG GTTGGAAAGT TGGCTGCAAC AGCTGTGGGA GGTGGATTTT 301 TTCTCCTTCA GCTTGCAAAC CATTCTGGGT ACATCAAAGT TGATTGGCAA CGAGTGGAGA 361 AGGACATGAA GAAAGCCAAA GAGCAGCTGA AGATCCCTAA GAGCACTCAG ATACCTAACC 421 AGGTCAGGAG CAAAGCTGAG GAGGTGGTGT CATTTGTGAA GAAAAATGTT CTAGTGACTG 481 GGGGATTTTT CGGAGGCTTT CTGCTTGGCA TGGCATCCTA CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGACATCAG TGCAGATACT CGCAGCCGAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt