Construct: ORF TRCN0000465591
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002521.1_s317c1
- Derived from:
- ccsbBroadEn_08053
- DNA Barcode:
- TCGGGACCCTACAGAATTAAACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAF5L (27097)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465591
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27097 | TAF5L | TATA-box binding protein as... | NM_001025247.2 | 99.7% | 100% | 678C>T;744A>C |
| 2 | human | 27097 | TAF5L | TATA-box binding protein as... | NM_014409.3 | 54.9% | 55% | (many diffs) |
| 3 | human | 27097 | TAF5L | TATA-box binding protein as... | XM_005273099.4 | 54.9% | 55% | (many diffs) |
| 4 | human | 27097 | TAF5L | TATA-box binding protein as... | XM_011544162.1 | 41.7% | 40.5% | (many diffs) |
| 5 | human | 27097 | TAF5L | TATA-box binding protein as... | XM_005273100.2 | 39.3% | 39.3% | (many diffs) |
| 6 | human | 27097 | TAF5L | TATA-box binding protein as... | XM_011544163.1 | 39.3% | 39.3% | (many diffs) |
| 7 | human | 27097 | TAF5L | TATA-box binding protein as... | XM_011544164.2 | 39.3% | 39.3% | (many diffs) |
| 8 | human | 27097 | TAF5L | TATA-box binding protein as... | XM_017001045.1 | 39.3% | 39.3% | (many diffs) |
| 9 | mouse | 102162 | Taf5l | TATA-box binding protein as... | NM_133966.2 | 47.6% | 50.9% | (many diffs) |
| 10 | mouse | 102162 | Taf5l | TATA-box binding protein as... | XM_006530546.3 | 47.6% | 50.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa acgagtgcgt accgagcaga ttcagatggc agtgtcctgc tacctcaaac 121 gccggcagta cgtggactca gatggtcccc tgaagcaagg actgcggctg tcacagactg 181 ctgaagagat ggcggccaat ctaacagtgc aatcagaatc tggttgtgcc aacatagtgt 241 ctgcagcccc ttgccaggca gaaccccagc aatatgaagt acagtttgga cgactgcgga 301 attttctcac tgattctgat tcccagcata gccacgaagt gatgcctctc ctctatcctc 361 tctttgtcta cctccatctc aacctggtcc aaaacagtcc gaagagcaca gtggaaagtt 421 tttacagccg cttccatgga atgtttctgc agaatgctag ccagaaggat gtcattgagc 481 agctacagac cactcaaacc atccaggaca tcctatctaa cttcaagctt cgagcattcc 541 tagataacaa gtacgtggtc cgtctccaag aagacagcta caactacctt atccgctacc 601 tccaaagtga caacaatact gccctgtgca aagtcctcac cttacatatt catcttgacg 661 tgcagcctgc caagagaaca gactatcagc tgtatgccag tggcagctcc tcccgcagtg 721 agaacaacgg tttGGAGCCC CCTGACATGC CCAGCCCTAT TCTGCAGAAC GAGGCTGCCC 781 TAGAGGTCTT ACAGGAGAGC ATTAAGCGCG TCAAGGATGG GCCTCCCTCC CTCACTACCA 841 TCTGCTTCTA TGCCTTCTAT AACACAGAGC AGCTGTTGAA CACTGCAGAA ATCTCCCCCG 901 ATAGCAAGCT GCTTGCTGCT GGGTTTGACA ACTCCTGTAT AAAACTTTGG AGTTTACGAT 961 CCAAGAAGTT AAAATCAGAG CCCCACCAAG TAGACGTGTC CCGCATCCAT TTGGCTTGTG 1021 ATATTCTGGA GGAGGAGGTA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1081 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1141 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCGG GACCCTACAG 1201 AATTAAACAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt