Construct: ORF TRCN0000465648
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004462.1_s317c1
- Derived from:
- ccsbBroadEn_04259
- DNA Barcode:
- CAGCGGCAAACTCAGCTGAACCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM14B (81853)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465648
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81853 | TMEM14B | transmembrane protein 14B | NM_030969.5 | 100% | 100% | |
| 2 | human | 51522 | TMEM14C | transmembrane protein 14C | NM_001165258.1 | 88% | 73.6% | (many diffs) |
| 3 | human | 51522 | TMEM14C | transmembrane protein 14C | NM_016462.4 | 88% | 73.6% | (many diffs) |
| 4 | human | 81853 | TMEM14B | transmembrane protein 14B | NM_001286488.1 | 71.5% | 67.5% | (many diffs) |
| 5 | human | 81853 | TMEM14B | transmembrane protein 14B | NM_001127711.2 | 70.1% | 70.1% | 98_99ins102 |
| 6 | human | 81853 | TMEM14B | transmembrane protein 14B | NM_001286484.1 | 68.6% | 65.8% | 1_44del;67_68ins77 |
| 7 | human | 81853 | TMEM14B | transmembrane protein 14B | NM_001286489.1 | 49.1% | 45% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 408
- ORF length:
- 342
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gaagcccctc ttcccattag tgcctttgca ttggtttggc tttggctaca 121 cagcactggt tgtttctggt gggatcgttG GCTATGTAAA AACAGGCAGC GTGCCGTCCC 181 TGGCTGCAGG GCTGCTCTTC GGCAGTCTAG CCGGCCTGGG TGCTTACCAG CTGTATCAGG 241 ATCCAAGGAA CGTTTGGGGT TTCCTAGCCG CTACATCTGT TACTTTTGTT GGTGTTATGG 301 GAATGAGATC CTACTACTAT GGAAAATTCA TGCCTGTAGG TTTAATTGCA GGTGCCAGTT 361 TGCTGATGGC CGCCAAAGTT GGAGTTCGTA TGTTGATGAC ATCTGATTAC CCAACTTTCT 421 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 481 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 541 GTGGAAAGGA CGACAGCGGC AAACTCAGCT GAACCGAACG CGTTAAGTCg acaatcaacc 601 tctggattac aaaatttgtg aaagatt