Construct: ORF TRCN0000465648

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004462.1_s317c1
Derived from:
ccsbBroadEn_04259
DNA Barcode:
CAGCGGCAAACTCAGCTGAACCGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM14B (81853)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000465648
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81853 TMEM14B transmembrane protein 14B NM_030969.5 100% 100%
2 human 51522 TMEM14C transmembrane protein 14C NM_001165258.1 88% 73.6% (many diffs)
3 human 51522 TMEM14C transmembrane protein 14C NM_016462.4 88% 73.6% (many diffs)
4 human 81853 TMEM14B transmembrane protein 14B NM_001286488.1 71.5% 67.5% (many diffs)
5 human 81853 TMEM14B transmembrane protein 14B NM_001127711.2 70.1% 70.1% 98_99ins102
6 human 81853 TMEM14B transmembrane protein 14B NM_001286484.1 68.6% 65.8% 1_44del;67_68ins77
7 human 81853 TMEM14B transmembrane protein 14B NM_001286489.1 49.1% 45% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
408
ORF length:
342
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga gaagcccctc ttcccattag tgcctttgca ttggtttggc tttggctaca
121cagcactggt tgtttctggt gggatcgttG GCTATGTAAA AACAGGCAGC GTGCCGTCCC
181TGGCTGCAGG GCTGCTCTTC GGCAGTCTAG CCGGCCTGGG TGCTTACCAG CTGTATCAGG
241ATCCAAGGAA CGTTTGGGGT TTCCTAGCCG CTACATCTGT TACTTTTGTT GGTGTTATGG
301GAATGAGATC CTACTACTAT GGAAAATTCA TGCCTGTAGG TTTAATTGCA GGTGCCAGTT
361TGCTGATGGC CGCCAAAGTT GGAGTTCGTA TGTTGATGAC ATCTGATTAC CCAACTTTCT
421TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
481CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
541GTGGAAAGGA CGACAGCGGC AAACTCAGCT GAACCGAACG CGTTAAGTCg acaatcaacc
601tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)