Construct: ORF TRCN0000465688
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015330.1_s317c1
- Derived from:
- ccsbBroadEn_12158
- DNA Barcode:
- CACCGACAGTTACGCGATTAAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGGF1 (55109)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465688
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55109 | AGGF1 | angiogenic factor with G-pa... | NM_018046.5 | 12.7% | 10.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 393
- ORF length:
- 327
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcggaggcg ccgtccccgc cgcggtcgcc gccgccgccc acctcccccg 121 agccTGAGCT GGCCCAGCTA AGGCGGAAGG TGGAGAAGTT GGAACGTGAA CTGCGGAGCT 181 GCAAGCGGCA GGTGCGGGAG ATCGAGAAGC TGCTGCATCA CACAGAACGG CTGTACCAGA 241 ACGCAGAAAG CAACAACCAG GAGCTCCGCA CGCAGGTGCG CGGTCCTCCT CAGCCCCGCG 301 CCCCATCCAG CCCAGGCGAG GCCTTCGAAG CCCGTGATAG CCTCGGAAGG GGTCCCTGGC 361 AGGGGCTCAG AACTACTGTA GAGTACTTAA AGTACCCAAC TTTCTTGTAC AAAGTGGTTG 421 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 481 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 541 CCGACAGTTA CGCGATTAAA TAACGCGTTA AGTCgacaat caacctctgg attacaaaat 601 ttgtgaaaga tt