Construct: ORF TRCN0000465694
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002628.1_s317c1
- Derived from:
- ccsbBroadEn_15428
- DNA Barcode:
- CGGCCTGCCCCGTCGATACGATCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GCLC (2729)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465694
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2729 | GCLC | glutamate-cysteine ligase c... | NM_001498.4 | 39.5% | 39.5% | 756_1911delinsA |
| 2 | human | 2729 | GCLC | glutamate-cysteine ligase c... | NM_001197115.2 | 33.5% | 33.5% | 443_444ins114;642_1797delinsA |
| 3 | mouse | 14629 | Gclc | glutamate-cysteine ligase, ... | NM_010295.2 | 34.7% | 36.5% | (many diffs) |
| 4 | mouse | 14629 | Gclc | glutamate-cysteine ligase, ... | XM_006510812.1 | 27% | 28.4% | (many diffs) |
| 5 | mouse | 14629 | Gclc | glutamate-cysteine ligase, ... | XR_001778796.1 | 18.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 822
- ORF length:
- 756
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gctgctgtcc cagggctcgc cgctgagctg ggaggaaacc aagcgccatg 121 ccgaccacgt gcggcggcac gggatcctcc agttcctgca catctaccac gccgtcaagg 181 accggcacaa ggacgttctc aagtggggcg atgaggtgga atacatgttg gtatcttttg 241 atcatgaaaa taaaaaagtc cggttggtcc tgtctgggga gaaagttctt gaaactctgc 301 aagagaaggg ggaaaggaca aacccaaacc atcctaccct ttggagacca gagtatggga 361 gttacatgat tgaagggaca ccaggacagc ccTACGGAGG AACAATGTCC GAGTTCAATA 421 CAGTTGAGGC CAACATGCGA AAACGCCGGA AGGAGGCTAC TTCTATATTA GAAGAAAATC 481 AGGCTCTTTG CACAATAACT TCATTTCCCA GATTAGGCTG TCCTGGGTTC ACACTGCCCG 541 AGGTCAAACC CAACCCAGTG GAAGGAGGAG CTTCCAAGTC CCTCTTCTTT CCAGATGAAG 601 CAATAAACAA GCACCCTCGC TTCAGTACCT TAACAAGAAA TATCCGACAT AGGAGAGGAG 661 AAAAGGTTGT CATCAATGTA CCAATATTTA AGGACAAGAA TACACCATCT CCATTTATAG 721 AAACATTTAC TGAGGATGAT GAAGCTTCAA GGGCTTCTAA GCCGGATCAT ATTTACATGG 781 ATGCCATGGG ATTTGGAATG GGCAATTGCT GTCTCCAGGT ATACCCAACT TTCTTGTACA 841 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 901 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 961 AGGACGACGG CCTGCCCCGT CGATACGATC AACGCGTTAA GTCgacaatc aacctctgga 1021 ttacaaaatt tgtgaaagat t