Construct: ORF TRCN0000465768
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008000.1_s317c1
- Derived from:
- ccsbBroadEn_10295
- DNA Barcode:
- TGTTCATCCGGCATGCGCCTTGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARHGAP27P1 (146880)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465768
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 146880 | ARHGAP27P1 | Rho GTPase activating prote... | NR_027487.2 | 24.5% | 1_319del;1031_2891del | |
| 2 | human | 109286553 | ARHGAP27P1-BPTFP1-KPNA2P3 | ARHGAP27P1-BPTFP1-KPNA2P3 r... | NR_026899.1 | 21.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ctccccgcga acgggaacgc ccgcactaga caaccagccc cagaccggca 121 ttcccagagc cgcaggcggg agggacgcag ggactccggg acagcaaatc cccgggggag 181 aagggaaggg ggaaggcggg acaggggagg cccaccctcc ttgggctccg ctagcgacca 241 gccagcgccc cttcagacgc agccttcctg gctccggacc ccggctttct gctccggtcc 301 tcgtcccaat gccccccaag gtccccagca gaggcattca ggttcctcct cgccccgccc 361 agacgcagct tctttgggta tgggtgacag cactccTGAG CGTCGCTGTC CCTATTCGCT 421 GCCGTTGCCA GCTCCGATTC CGCGAAACCT CTCCGGCACC ACCTCGGAAG CCCCGCCCAG 481 GGCGATGGTC CGGGCCTAAC CTGCCGGCCC CGCCCACCCG GCCCTTGGGC TCCCTGAGGC 541 CCGCCCAGCA GACTCGAGGA ACGCTTGACA AAGTGGCGAA GGAGCCCTAC GGGAGTCCCG 601 GATTGGACCC TGCTCCTTCG GTCCCTCAGC CTCGGCAAGC CGCGGTCTCC TGGCCCTTGG 661 CCTGGGTCAC CTTAGGAGGC AGGAGGAGAT GTGGTCAGTG TGGGAGCTTG GAGGGGCCAT 721 GCACTTCCGG AGAAGACCAC CGACTCATGG CTTTGGTGGA GGGGCGCCGG CAGATTTACC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GATGTTCATC CGGCATGCGC CTTGCGACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt