Construct: ORF TRCN0000465770
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003830.1_s317c1
- Derived from:
- ccsbBroadEn_06222
- DNA Barcode:
- ACATTGCAGAAACTAGATCAATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FXN (2395)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465770
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2395 | FXN | frataxin | NM_000144.5 | 99.8% | 100% | 54A>G |
| 2 | human | 2395 | FXN | frataxin | NM_181425.3 | 90.7% | 72.6% | 54A>G;481_488delAGGTATGT;588_589ins50 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 696
- ORF length:
- 630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg gactctcggg cgccgcgcag tagccggcct cctggcgtca cccagcccgg 121 cccaggccca gaccctcacc cgggtcccgc ggccggcaga gttggcccca ctctgcggcc 181 gccgtggcct gcgcaccgac atcgatgcga cctgcacgcc ccgccgcgca agttcgaacc 241 aacgtggcct caaccagatt tggaatgtca aaaagcagag tgtctatttg atgaattTGA 301 GGAAATCTGG AACTTTGGGC CACCCAGGCT CTCTAGATGA GACCACCTAT GAAAGACTAG 361 CAGAGGAAAC GCTGGACTCT TTAGCAGAGT TTTTTGAAGA CCTTGCAGAC AAGCCATACA 421 CGTTTGAGGA CTATGATGTC TCCTTTGGGA GTGGTGTCTT AACTGTCAAA CTGGGTGGAG 481 ATCTAGGAAC CTATGTGATC AACAAGCAGA CGCCAAACAA GCAAATCTGG CTATCTTCTC 541 CATCCAGTGG ACCTAAGCGT TATGACTGGA CTGGGAAAAA CTGGGTGTAC TCCCACGACG 601 GCGTGTCCCT CCATGAGCTG CTGGCCGCAG AGCTCACTAA AGCCTTAAAA ACCAAACTGG 661 ACTTGTCTTC CTTGGCCTAT TCCGGAAAAG ATGCTTGCCC AACTTTCTTG TACAAAGTGG 721 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 781 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 841 AACATTGCAG AAACTAGATC AATGCACGCG TTAAGTCgac aatcaacctc tggattacaa 901 aatttgtgaa agatt