Construct: ORF TRCN0000465772
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006407.1_s317c1
- Derived from:
- ccsbBroadEn_06601
- DNA Barcode:
- AATCGTTACTAGACGAATTGACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTAP (4507)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465772
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4507 | MTAP | methylthioadenosine phospho... | NM_002451.4 | 99.6% | 99.6% | 166G>A;438C>A;561C>T |
| 2 | mouse | 66902 | Mtap | methylthioadenosine phospho... | NM_024433.2 | 87.5% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 915
- ORF length:
- 849
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctctggcacc accaccaccg ccgtgaagat tggaataatt ggtggaacag 121 gcctggatga tccagaaatt ttagaaggaa gaactgaaaa atatgtggat actccatttg 181 gcaagccatc tgatgcctta attttgggga agataaaaaa tgttgattgc atcctccttg 241 caaggcatgg aaggcagcac accatcatgc cttcaaaggt caactaccag gcgaacatct 301 gggctttgaa ggaagagggc tgtacacatg tcatagtgac cacagcttgt ggctccttga 361 gggaggagat tcagcccggc gatattgtca ttattgatca gttcattgac aggaccacta 421 tgagacctca gtccttctat gatggaagtc attcttgtgc cagaggagtg tgccatattc 481 caatggctga gccgttttgc ccaaaaacga gagaggttct tatagagact gctaagaagc 541 taggactccg gtgccactca aaggggacaa tggtcacaat cgagggacct cgttttagct 601 cccgggcaga aagcttcatg ttccgtacct ggggggcgga tgttatcaac atgaccacag 661 ttccagaggt ggttcttgct aaggaggctg gaatttgtta cgcaagtatc gccatggcga 721 cagattatga ctgctggaag gagcacgagg aagcagtttc ggtggaccgg gtcttaaaga 781 ccctgaaaga aaacgctaat aaagccaaaa gcttactgct cactaccata ccTCAGATAG 841 GGTCCACAGA ATGGTCAGAA ACCCTCCATA ACCTGAAGAA TATGGCCCAG TTTTCTGTTT 901 TATTACCAAG ACATTACCTA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 961 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGCCCGTAA CTTGAAAGTA 1021 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA AATCGTTACT AGACGAATTG 1081 ACAAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt