Construct: ORF TRCN0000465805
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012412.1_s317c1
- Derived from:
- ccsbBroadEn_07143
- DNA Barcode:
- GAAAACATACACAAGTGTTAAGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFP36 (7538)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465805
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7538 | ZFP36 | ZFP36 ring finger protein | NM_003407.5 | 100% | 100% | |
| 2 | mouse | 22695 | Zfp36 | zinc finger protein 36 | NM_011756.4 | 82.8% | 83.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1044
- ORF length:
- 978
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tctgactgcc atctacgaga gcctcctgtc gctgagccct gacgtgcccg 121 tgccatccga ccatggaggg actgagtcca gcccaggctg gggctcctcg ggaccctgga 181 gcctgagccc ctccgactcc agcccgtctg gggtcacctc ccgcctgcct ggccgctcca 241 ccagcctagt ggagggccgc agctgtggct gggtgccccc accccctggc ttcgcaccgc 301 tggctccccg cctgggccct gagctgtcac cctcacccac ttcgcccact gcaacctcca 361 ccaccccctc gcgctacaag actgagctat gtcggacctt ctcagagagt gggcgctgcc 421 gctacggggc caagtgccag tttgcccatg gcctgggcga gctgcgccag gccaatcgcc 481 accccaaata caagacggaa ctctgtcaca agttctacct ccagggccgc tgcccctacg 541 gctctcgctg ccacttcatc cacaacccta gcgaagacct ggcggccccg ggccaccctc 601 ctgtgcttcg ccagagcatc agcttctccg gcctgccctc tggccgccgg acctcaccac 661 caccaccagg cctggccggc ccttccctgt cctccagctc cttctcgccc tccagctccc 721 caccaccacc tggggacctt ccactgtcac cctctgcctt cTCTGCTGCC CCTGGCACCC 781 CCCTGGCTCG AAGAGACCCC ACCCCAGTCT GTTGCCCCTC CTGCCGAAGG GCCACTCCTA 841 TCAGCGTCTG GGGGCCCTTG GGTGGCCTGG TTCGGACCCC CTCTGTACAG TCCCTGGGAT 901 CCGACCCTGA TGAATATGCC AGCAGCGGCA GCAGCCTGGG GGGCTCTGAC TCTCCCGTCT 961 TCGAGGCGGG AGTTTTTGCA CCACCCCAGC CCGTGGCAGC CCCCCGGCGA CTCCCCATCT 1021 TCAATCGCAT CTCTGTTTCT GAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1081 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1141 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG AAAACATACA 1201 CAAGTGTTAA GTAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1261 att