Construct: ORF TRCN0000465834
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006630.1_s317c1
- Derived from:
- ccsbBroadEn_01457
- DNA Barcode:
- TTTAGGACTAAAAGTTTGTCTACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS19 (6223)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465834
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6223 | RPS19 | ribosomal protein S19 | NM_001022.4 | 100% | 100% | |
| 2 | human | 6223 | RPS19 | ribosomal protein S19 | NM_001321483.2 | 100% | 100% | |
| 3 | human | 6223 | RPS19 | ribosomal protein S19 | NM_001321484.2 | 100% | 100% | |
| 4 | human | 6223 | RPS19 | ribosomal protein S19 | NM_001321485.2 | 92.9% | 41.8% | 172_184del;449_468del |
| 5 | human | 6223 | RPS19 | ribosomal protein S19 | XM_017027113.2 | 74.3% | 72% | (many diffs) |
| 6 | mouse | 20085 | Rps19 | ribosomal protein S19 | NM_023133.1 | 93.3% | 99.3% | (many diffs) |
| 7 | mouse | 20085 | Rps19 | ribosomal protein S19 | XM_006539680.2 | 93.3% | 99.3% | (many diffs) |
| 8 | mouse | 20085 | Rps19 | ribosomal protein S19 | XM_006539681.2 | 93.3% | 99.3% | (many diffs) |
| 9 | mouse | 277692 | Rps19-ps3 | ribosomal protein S19, pseu... | NR_033639.1 | 88.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tggagttact gtaaaagacg tgaaccagca ggagttcgtc agagctctgg 121 cagccttcct caaaaagtcc gggaagctga aagtccccga atgggtggat accgtcaagc 181 tggccaagca caaagagctt gctccctacg atgagaactg gttctacacg cgagctgctt 241 ccacagcgcg gcacctgtac cTCCGGGGTG GCGCTGGGGT TGGCTCCATG ACCAAGATCT 301 ATGGGGGACG TCAGAGAAAC GGCGTCATGC CCAGCCACTT CAGCCGAGGC TCCAAGAGTG 361 TGGCCCGCCG GGTCCTCCAA GCCCTGGAGG GGCTGAAAAT GGTGGAAAAG GACCAAGATG 421 GCGGCCGCAA ACTGACACCT CAGGGACAAA GAGATCTGGA CAGAATCGCC GGACAGGTGG 481 CAGCTGCCAA CAAGAAGCAT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTTA GGACTAAAAG 661 TTTGTCTACT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt