Construct: ORF TRCN0000465936
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006063.1_s317c1
- Derived from:
- ccsbBroadEn_15440
- DNA Barcode:
- GTTTATCCTTCCCCACGTGGATCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HBG1 (3047)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465936
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3047 | HBG1 | hemoglobin subunit gamma 1 | NM_000559.2 | 99.7% | 97.9% | 434delG |
| 2 | human | 3048 | HBG2 | hemoglobin subunit gamma 2 | NM_000184.3 | 99.3% | 96.5% | 227T>C;410G>C;434delG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tcatttcaca gaggaggaca aggctactat cacaagcctg tggGGCAAGG 121 TGAATGTGGA AGATGCTGGA GGAGAAACCC TGGGAAGGCT CCTGGTTGTC TACCCATGGA 181 CCCAGAGGTT CTTTGACAGC TTTGGCAACC TGTCCTCTGC CTCTGCCATC ATGGGCAACC 241 CCAAAGTCAA GGCACATGGC AAGAAGGTGC TGACTTCCTT GGGAGATGCC ACAAAGCACC 301 TGGATGATCT CAAGGGCACC TTTGCCCAGC TGAGTGAACT GCACTGTGAC AAGCTGCATG 361 TGGATCCTGA GAACTTCAAG CTCCTGGGAA ATGTGCTGGT GACCGTTTTG GCAATCCATT 421 TCGGCAAAGA ATTCACCCCT GAGGTGCAGG CTTCCTGGCA GAAGATGGTG ACTGCAGTGG 481 CCAGTGCCCT GTCCTCCAAT ACCACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 541 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 601 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG AGTTTATCCT 661 TCCCCACGTG GATCCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 721 agatt