Construct: ORF TRCN0000465968
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003520.1_s317c1
- Derived from:
- ccsbBroadEn_04641
- DNA Barcode:
- CAGTTTAGCAGGTGGAAATGCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCAMP4 (113178)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000465968
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 113178 | SCAMP4 | secretory carrier membrane ... | NM_079834.4 | 100% | 100% | |
| 2 | human | 113178 | SCAMP4 | secretory carrier membrane ... | NM_001329540.2 | 85.1% | 85.1% | 292_293ins102 |
| 3 | human | 113178 | SCAMP4 | secretory carrier membrane ... | NM_001329539.2 | 80.7% | 80.7% | 0_1ins132 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 753
- ORF length:
- 687
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc agaaaaggag aacaacttcc cgccactgcc caagttcatc cctgtgaagc 121 cctgcttcta ccagaacttc tccgacgaga tcccagtgga gcaccaggtc ctggtgaaga 181 ggatctaccg gctgtggatg ttttactgcg ccaccctcgg cgtcaacctc attgcctgcc 241 tggcctggtg gatcggcgga ggctcgggga ccaacttcgg cctggccttc gtgtggctgc 301 tcctgttcac gccttgcggc tacgtgtgct ggttccggcc tgtctacaag gCCTTCCGAG 361 CCGACAGCTC CTTTAATTTC ATGGCGTTTT TCTTCATCTT CGGAGCCCAG TTTGTCCTGA 421 CCGTCATCCA GGCGATTGGC TTCTCCGGCT GGGGCGCGTG CGGCTGGCTG TCGGCAATTG 481 GATTCTTCCA GTACAGCCCG GGCGCTGCCG TGGTCATGCT GCTTCCAGCC ATCATGTTCT 541 CCGTGTCGGC TGCCATGATG GCCATCGCGA TCATGAAGGT GCACAGGATC TACCGAGGGG 601 CTGGCGGAAG CTTCCAGAAG GCACAGACGG AGTGGAACAC GGGCACTTGG CGGAACCCAC 661 CGTCGAGGGA GGCCCAGTAC AACAACTTCT CAGGCAACAG CCTGCCCGAG TACCCCACTG 721 TGCCCAGCTA CCCGGGCAGT GGCCAGTGGC CTTACCCAAC TTTCTTGTAC AAAGTGGTTG 781 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 841 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACA 901 GTTTAGCAGG TGGAAATGCT TGACGCGTTA AGTCgacaat caacctctgg attacaaaat 961 ttgtgaaaga tt