Construct: ORF TRCN0000466002
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004226.1_s317c1
- Derived from:
- ccsbBroadEn_00687
- DNA Barcode:
- CAAGCATGCTGAACCCAGTTAGAA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GPX4 (2879)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466002
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_002085.5 | 100% | 100% | |
| 2 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_001039847.3 | 86.7% | 100% | 500_521del;614_681del |
| 3 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_001367832.1 | 86.2% | 62.5% | 0_1ins81 |
| 4 | human | 2879 | GPX4 | glutathione peroxidase 4 | NM_001039848.4 | 80% | 43.4% | (many diffs) |
| 5 | mouse | 625249 | Gpx4 | glutathione peroxidase 4 | NM_008162.3 | 86.4% | 95.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 282
- ORF length:
- 216
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag cctcggccgc ctttgccgcc tactgaagcc ggcgctgctc tgtggggctc 121 tggccgcgcc tggcctggcc gggaccatgt gcgcgtcccg ggacgactgg cgctgtgcgc 181 gctccatgca cgagttttcc gccaaggaca tcgacgggca catggttaac ctggacaagt 241 accggggctt cgtgtgcatc gtcaccaacg tggccTCCCA GTGAGGCAAG ACCGAAGTAA 301 ACTACACTCA GCTCGTCGAC CTGCACGCCC GATACGCTGA GTGTGGTTTG CGGATCCTGG 361 CCTTCCCGTG TAACCAGTTC GGGAAGCAGG AGCCAGGGAG TAACGAAGAG ATCAAAGAGT 421 TCGCCGCGGG CTACAACGTC AAATTCGATA TGTTCAGCAA GATCTGCGTG AACGGGGACG 481 ACGCCCACCC GCTGTGGAAG TGGATGAAGA TCCAACCCAA GGGCAAGGGC ATCCTGGGAA 541 ATGCCATCAA GTGGAACTTC ACCAAGTTCC TCATCGACAA GAACGGCTGC GTGGTGAAGC 601 GCTACGGACC CATGGAGGAG CCCCTGGTGA TAGAGAAGGA CCTGCCCCAC TATTTCTACC 661 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 721 TCGATTCTAC GTAGTAATGA ACTAGCCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 781 ATATATCTTG TGGAAAGGAC GACAAGCATG CTGAACCCAG TTAGAAACGC GTTAAGTCga 841 caatcaacct ctggattaca aaatttgtga aagatt