Construct: ORF TRCN0000466025
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007551.1_s317c1
- Derived from:
- ccsbBroadEn_01629
- DNA Barcode:
- AGAGCGCAATACTCACCCAACCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VAMP2 (6844)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466025
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6844 | VAMP2 | vesicle associated membrane... | NM_014232.3 | 100% | 100% | |
| 2 | human | 6844 | VAMP2 | vesicle associated membrane... | NM_001330125.1 | 98% | 97.4% | 1_6delATGGAC;8G>T |
| 3 | mouse | 22318 | Vamp2 | vesicle-associated membrane... | NM_009497.3 | 94.2% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 414
- ORF length:
- 348
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgctaccgct gccacggccc cccctgctgc cccggctggg gagggtggTC 121 CCCCTGCACC CCCTCCAAAC CTCACCAGTA ACAGGAGACT GCAGCAGACC CAGGCCCAGG 181 TGGATGAGGT GGTGGACATC ATGAGGGTGA ACGTGGACAA GGTCCTGGAG CGAGACCAGA 241 AGCTGTCGGA GCTGGACGAC CGTGCAGATG CACTCCAGGC GGGGGCCTCC CAGTTTGAAA 301 CAAGCGCAGC CAAGCTCAAG CGCAAATACT GGTGGAAAAA CCTCAAGATG ATGATCATCT 361 TGGGAGTGAT TTGCGCCATC ATCCTCATCA TCATCATAGT TTACTTCAGC ACTTACCCAA 421 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 481 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 541 TATCTTGTGG AAAGGACGAA GAGCGCAATA CTCACCCAAC CTCACGCGTT AAGTCgacaa 601 tcaacctctg gattacaaaa tttgtgaaag att