Construct: ORF TRCN0000466031
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011133.1_s317c1
- Derived from:
- ccsbBroadEn_07918
- DNA Barcode:
- ATCATTCTCTAGATTTTGCGCAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CBLC (23624)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466031
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23624 | CBLC | Cbl proto-oncogene C | NM_012116.4 | 99.9% | 99.7% | 1237T>C |
| 2 | human | 23624 | CBLC | Cbl proto-oncogene C | XM_011526688.2 | 93.9% | 91.8% | (many diffs) |
| 3 | human | 23624 | CBLC | Cbl proto-oncogene C | XM_005258696.3 | 90.3% | 90% | 1237T>C;1285_1288delAACA;1293_1294ins133 |
| 4 | human | 23624 | CBLC | Cbl proto-oncogene C | NM_001130852.1 | 90.2% | 90% | 779_780ins138;1099T>C |
| 5 | human | 23624 | CBLC | Cbl proto-oncogene C | XM_011526689.2 | 84.7% | 82.6% | (many diffs) |
| 6 | human | 23624 | CBLC | Cbl proto-oncogene C | XR_935783.2 | 84.2% | (many diffs) | |
| 7 | human | 23624 | CBLC | Cbl proto-oncogene C | XR_243917.3 | 69.4% | 1_73del;991_1006del;1138_1139ins373 | |
| 8 | human | 23624 | CBLC | Cbl proto-oncogene C | XM_011526690.2 | 57.9% | 49.2% | 654_655ins122;796_811del;849_850ins467 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1488
- ORF length:
- 1422
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tctggcggtg gccccgtggg ggcgacagtg ggaagaggcc cgcgccctgg 121 gccgggcagt caggatgctg cagcgcctag aagagcaatg cgtcgacccc cggctgtccg 181 tgagtccccc ttcgctgcgg gacctgctgc cccgcacagc gcagctgctt cgagaggtgg 241 cccattctcg gcgggcggcc ggcggaggcg gccccggggg tcccggcggc tctggggact 301 ttctactcat ctacctggcc aatctggagg ccaagagcag gcaggtggcc gcgctgctgc 361 ctccccgggg ccgaaggagt gccaacgacg agctcttccg ggcgggctcc agactcaggc 421 gacagctggc caagctggcc atcatcttca gccacatgca cgcagagctg cacgcactct 481 tccccggggg aaagtactgt ggacacatgt accagctcac caaggccccc gcccacacct 541 tctggaggga aagttgcgga gcccggtgtg tgctgccctg ggctgagttt gagtccctcc 601 tgggcacctg ccaccctgtg gaaccaggct gcacagccct ggccttgcgc accaccattg 661 acctcacctg cagcgggcac gtgtccatct tcgagttcga cgtcttcacc aggctctttc 721 agccatggcc aacactcctc aagaactggc agctcctggc agtcaaccac ccaggctaca 781 tggccttcct cacctatgat gaggtccaag agcgtctgca ggcctgcagg gacaagccag 841 gcagttacat cttccggccc agctgtactc gcctggggca gtgggccatc ggctatgtga 901 gctcagatgg cagcatcctg cagaccatcc ctgccaacaa acccctgtcc caggtgctcc 961 tggagggaca gaaggacggc ttctacctct acccagatgg aaagacccac aacccagacc 1021 tgactgagct cggccaggca gaaccccagc agcgcatcca cgtgtcagag gagcagctgc 1081 agctctactg ggccatggac tccacatttG AGCTCTGCAA GATCTGTGCT GAGAGCAACA 1141 AGGATGTGAA GATTGAGCCG TGCGGGCACC TGCTCTGCAG CTGCTGCCTG GCTGCCTGGC 1201 AGCACTCGGA CAGCCAGACC TGCCCCTTCT GCCGCTGCGA GATCAAGGGC TGGGAGGCCG 1261 TGAGTATCTA CCAGTTCCAC GGTCAGGCTA CTGCTGAGGA CCCAGGGAAC AGCAGTGACC 1321 AGGAAGGCAG GGAGTTGGAG CTGGGGCAGG TGCCCCTTTC GGCTCCTCCA TTGCCCCCAC 1381 GGCCAGATCT GCCCCCCAGG AAGCCCAGAA ATGCCCAGCC GAAAGTGAGA CTCCTAAAGG 1441 GGAACTCCCC TCCAGCTGCG CTGGGACCCC AGGACCCTGC CCCGGCCTGC CCAACTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGAATCATTC TCTAGATTTT GCGCAAAACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt