Construct: ORF TRCN0000466050
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005361.1_s317c1
- Derived from:
- ccsbBroadEn_11436
- DNA Barcode:
- GCTAGTAGCCCTATGTAGAGAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRYZL1 (9946)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466050
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9946 | CRYZL1 | crystallin zeta like 1 | NM_145858.3 | 29% | 26% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 429
- ORF length:
- 339
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAAGGCTT ATATTTCCAA CAGAGTTCCA 121 CAGATGAAGA AATAACATTT GTATTTCAAG AAAAGGAAGA TCTTCCTGTT ACAGAGGATA 181 ACTTTGTGAA ACTTCAAGTT AAAGCTTGTG CTCTGAGCCA GATAAATACA AAGCTTCTGG 241 CAGAAATGAA GATGAAAAAG GATTTATTTC CTGTTGGGAG AGAAATTGCT GGAATTGTAT 301 TAGATGTTGG AAGCAAGGTA TCATTCTTTC AACCAGATGA TGAAGTAGTT GAAGTACTTT 361 CCCGGCTGGG TGCTGTGGCT CACGTCTGTA ATCCCAGCAC GTTGGGAGGC CAAGGTGGGC 421 GAATCACCTG CCCCACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCTAGT AGCCCTATGT AGAGAATCAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt