Construct: ORF TRCN0000466066
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001888.1_s317c1
- Derived from:
- ccsbBroadEn_13490
- DNA Barcode:
- CTCGATTCTATACCCGAAGACGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OFCC1 (266553)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466066
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 266553 | OFCC1 | orofacial cleft 1 candidate 1 | XM_017011612.1 | 92.7% | 92.7% | 1_54del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 762
- ORF length:
- 693
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagagagag aagtttcagc agaaggctct gaagcaaact aagcagaaga 121 aatccaagtc ggctgaattt ctgatggtta aagaagatag agaagctaca gaaggcactg 181 gaaatccagc ttttaacatg agcagtccag atctctcagc atgtcagact gcagaaaaga 241 aagttattag acacgacatg ccggatcgca cccttgcagc tcaccaacaa aaattcaggc 301 tgccagcctc tgcagaacca aaaggaaatg aatacggcag aaattacttt gacccactga 361 tggatgagga aataaaccca aggcagtgtg cgacggaggt cagcagagaa gatgacgata 421 gaattttcta taatcgatta acgaagcttt tTGATGAGAG CAGACAAGGA GAACCCCAAG 481 ATGAGAGTGG CCGGGAGGAG ACTCTGAATT CTGAAGCACC TGGGAGTTCC AATAAGAGTC 541 ACGAAATCCA CAAAGAAGCC AGCGAAGCCA CCACTGCTCA CCTTGAGGAG TTCCAAAGAT 601 CTCAGAAAAC TATAATTCTG CTTGGAAGTT CTCCATTAGA ACAGGAAATT AGATCTACTT 661 CATTGCATTG TATGGAGGAT GAGATGAGTC ATCCATGGAT ACTCTTGCTC AAAGTAACTG 721 CTGTAATCAG AAGCAGGAGG TACTATAGAG AGCAAAGATT CTTGCCAACT TTCTTGTACA 781 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 841 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 901 AGGACGACTC GATTCTATAC CCGAAGACGA CACGCGTTAA GTCgacaatc aacctctgga 961 ttacaaaatt tgtgaaagat t