Construct: ORF TRCN0000466095
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002172.1_s317c1
- Derived from:
- ccsbBroadEn_05021
- DNA Barcode:
- TATGGGCTTGTCCATTTTTCCTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOB3C (148932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466095
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 148932 | MOB3C | MOB kinase activator 3C | NM_201403.2 | 100% | 100% | |
| 2 | human | 148932 | MOB3C | MOB kinase activator 3C | NM_145279.4 | 80.5% | 80.5% | 1_156del |
| 3 | mouse | 100465 | Mob3c | MOB kinase activator 3C | NM_175308.4 | 90.1% | 97.6% | (many diffs) |
| 4 | mouse | 100465 | Mob3c | MOB kinase activator 3C | XM_011240381.1 | 90.1% | 97.6% | (many diffs) |
| 5 | mouse | 100465 | Mob3c | MOB kinase activator 3C | XM_011240382.2 | 90.1% | 97.6% | (many diffs) |
| 6 | mouse | 100465 | Mob3c | MOB kinase activator 3C | XM_011240380.1 | 58% | 62.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 717
- ORF length:
- 648
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccctgtgc ctgaagcagg tgttcgccaa ggacaagacg ttccggccgc 121 ggaagcgctt tgagccgggc acacagcgct ttgagctgta caagaaggca caggcctctc 181 tcaagtcggg cctggacctg cgcagtgtgg tgaggctacc acccggggag aacatcgacg 241 actggatcgc cgtgcacgtg gtggacttct tcaaccgcat caacctcatc tacggcacta 301 tggcggagcg ctgcagtgag accagctgcc cggTCATGGC CGGCGGGCCC CGCTACGAGT 361 ACCGCTGGCA GGACGAGCGC CAGTACCGGC GGCCCGCCAA GCTCTCTGCG CCGCGCTATA 421 TGGCATTGCT CATGGACTGG ATCGAAGGCC TCATCAACGA CGAAGAGGTC TTTCCCACGC 481 GTGTTGGAGT TCCCTTCCCT AAGAACTTCC AGCAGGTCTG CACCAAGATC CTGACCCGCC 541 TCTTCCGAGT CTTTGTCCAT GTCTACATCC ACCACTTCGA TAGCATCCTC AGCATGGGGG 601 CAGAGGCGCA CGTCAACACC TGCTACAAGC ACTTCTACTA CTTCATCCGC GAGTTCAGTC 661 TGGTGGACCA GCGGGAGCTG GAGCCACTGA GGGAGATGAC AGAGCGGATC TGCCACTTGC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GATATGGGCT TGTCCATTTT TCCTAAACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt