Construct: ORF TRCN0000466113
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014492.1_s317c1
- Derived from:
- ccsbBroadEn_04868
- DNA Barcode:
- TCACGTTAGTCCTCGCGCCAAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- M1AP (130951)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466113
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130951 | M1AP | meiosis 1 associated protein | NM_001321739.2 | 100% | 100% | |
| 2 | human | 130951 | M1AP | meiosis 1 associated protein | NM_138804.4 | 100% | 100% | |
| 3 | human | 130951 | M1AP | meiosis 1 associated protein | XM_011532550.2 | 100% | 100% | |
| 4 | human | 130951 | M1AP | meiosis 1 associated protein | XM_011532551.2 | 100% | 100% | |
| 5 | human | 130951 | M1AP | meiosis 1 associated protein | NM_001281296.1 | 99.2% | 99.2% | 1419_1420insAAGCATCCCTGC |
| 6 | human | 130951 | M1AP | meiosis 1 associated protein | XM_006711946.3 | 93.3% | 93.3% | 1_114del |
| 7 | human | 130951 | M1AP | meiosis 1 associated protein | XM_011532548.2 | 92.6% | 92.6% | 1_114del;1533_1534insAAGCATCCCTGC |
| 8 | human | 130951 | M1AP | meiosis 1 associated protein | XM_011532549.2 | 89.7% | 89.7% | 1_114del;1046_1047ins60 |
| 9 | human | 130951 | M1AP | meiosis 1 associated protein | XM_024452711.1 | 86.2% | 86.2% | 1073_1074ins207;1212_1213insAAGCATCCCTGC |
| 10 | human | 130951 | M1AP | meiosis 1 associated protein | XM_011532552.2 | 83% | 82.9% | 1_114del;709_710ins174 |
| 11 | human | 130951 | M1AP | meiosis 1 associated protein | XM_011532553.2 | 81.1% | 81.1% | 1_114del;1187_1188ins207 |
| 12 | human | 130951 | M1AP | meiosis 1 associated protein | NM_001281295.2 | 68.3% | 67.9% | (many diffs) |
| 13 | human | 130951 | M1AP | meiosis 1 associated protein | XM_011532554.2 | 53.4% | 52.2% | (many diffs) |
| 14 | human | 130951 | M1AP | meiosis 1 associated protein | XM_005264152.3 | 52.6% | 51.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1656
- ORF length:
- 1590
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tcctgggcga actactggta aagggccctc tactcacact cagattgacc 121 agcaacctcc acggcttctc attgtgcaca ttgctctacc gtcctgggct gacatctgca 181 ccaacctctg tgaggctctg cagaacttct tctctctagc ctgcagcttg atgggcccca 241 gccgcatgtc cctgttcagt ttatacatgg tacaagatca gcatgagtgc atcctccctt 301 ttgtgcaagt gaaagggaac tttgctaggt tgcagacctg catctcagaa ctccgcatgt 361 tacagagaga agggtgtttc agatcacaag gtgcttctct gcggctggca gtagaggatg 421 ggctccagca attcaaacaa tacagcagac atgtgaccac aagggcagct ctgacctata 481 cctccctgga gattactatt ctgacttctc agcctggaaa agaggtggtc aaacagttgg 541 aggaagggtt gaaagataca gacctagcca gagtcaggag gtttcaggtc gttgaggtca 601 caaagggaat cctagagcac gtggactcag cgtctcctgt tgaggatacc agcaatgatg 661 agagttctat tctgggaact gacattgacc ttcagactat agacaatgat atcgtcagca 721 tggagatttt cttcaaagcc tggctacata acagtggaac agaccaagaa caaatccatc 781 ttcttctttc ttcacagtgt ttcagcaaca tttccagacc cagagataat ccaatgtgtc 841 tgaaatgtga tctccaagag cgactgctct gcccatccct actcgctggc acagctgacg 901 gctccttgag aatggatgac cctaaaggag acttcatcac actctaccag atggcttccc 961 agtcatcggc ctctcattac aagctccaag tgatcaaggc tttaaaatct agcgggctct 1021 gcgagtcatt gacatatgga ctcccgttca tcctcagacc tacaagctgt tggcagctgg 1081 actgggatga gctggagaca aatcagcaac atttccatgc tttgtgtcac agcctgctga 1141 aaagggaatg gctgctgtta gccaaggggg aaccaccggg cccaggacac agccagagaa 1201 ttcctgccag caccttctat gtgatcatgc cgtcacactc cctcacactg ctggtaaagg 1261 cggtggccac gcgggaactg atgctgccca gcaccttccc ccTGCTACCT GAGGACCCAC 1321 ATGATGATAG CCTTAAGAAT GTGGAGAGCA TGCTGGACAG CCTGGAGCTG GAGCCCACCT 1381 ACAACCCCTT GCATGTTCAA AGCCACCTGT ACTCACACCT GAGCAGCATC TATGCCAAGC 1441 CTCAGGGGCG GCTCCACCCA CACTGGGAGA GCCGAGCTCC GAGAAAGCAT CCCTGCAAGA 1501 CTGGGCAGTT GCAGACCAAC CGAGCTCGAG CTACTGTGGC CCCCCTGCCT ATGACTCCTG 1561 TCCCAGGCAG AGCCTCCAAG ATGCCAGCAG CCAGCAAATC TTCCTCAGAT GCCTTCTTCC 1621 TGCCTTCAGA GTGGGAGAAG GATCCCTCAA GGCCCTACCC AACTTTCTTG TACAAAGTGG 1681 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1741 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1801 ATCACGTTAG TCCTCGCGCC AAGAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1861 aatttgtgaa agatt