Construct: ORF TRCN0000466147
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012358.1_s317c1
- Derived from:
- ccsbBroadEn_13562
- DNA Barcode:
- AATTGTTGAGCTTCTATCTGGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C8orf31 (286122)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466147
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286122 | C8orf31 | chromosome 8 open reading f... | NR_149322.1 | 9.3% | (many diffs) | |
| 2 | human | 286122 | C8orf31 | chromosome 8 open reading f... | NR_126398.1 | 8.8% | 1_405del;592_2110del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 276
- ORF length:
- 186
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGCTGAAAA GCCACACCAG AATTCCTGCA 121 ATTCAGTGAG GCAGCTCTTC AAAACCAAGC AGCTGGTGAC GCACAGGGAC AGAGGGGCCC 181 AGGGGCTGCT GGCTGCCAGG ACCACCGCAT TGCAGAGGTC TCCCTTGCAG CAAGAGATCT 241 GGGAGTCCAC AACGGCACTT GTTCTTCCAG GCAGGTACCC AACTTTCTTG TACAAAGTGG 301 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 361 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 421 AAATTGTTGA GCTTCTATCT GGTTCACGCG TTAAGTCgac aatcaacctc tggattacaa 481 aatttgtgaa agatt