Construct: ORF TRCN0000466216
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007561.1_s317c1
- Derived from:
- ccsbBroadEn_11014
- DNA Barcode:
- TTTGGCACTTAACCACGTTTAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- P2RY11 (5032)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466216
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5032 | P2RY11 | purinergic receptor P2Y11 | NM_002566.5 | 56.9% | 56.9% | 1_483del |
| 2 | human | 692312 | PPAN-P2RY11 | PPAN-P2RY11 readthrough | NM_001040664.3 | 26.8% | 26.8% | 1_1743del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 tcggcatgcc cacactcagc ttctcccacc tgaagaggcc gcagcagggg gcgggcaact 121 gcagcgtggc caggcccgag gcctgcatca agtgtctggg gacagcagac cacgggctgg 181 cggcctacag agcgtatagc ctggtgctgg cggggttggg ctgcggcctg ccgctgctgc 241 tcacgctggc agcctacggc gccctcgggc gggccgtgcT ACGCAGCCCA GGCATGACTG 301 TGGCCGAGAA GCTGCGTGTG GCAGCGTTGG TGGCCAGTGG TGTGGCCCTC TACGCCAGCT 361 CCTATGTGCC CTACCACATC ATGCGGGTGC TCAACGTGGA TGCTCGGCGG CGCTGGAGCA 421 CCCGCTGCCC GAGCTTTGCA GACATAGCCC AGGCCACAGC AGCCCTGGAG CTGGGGCCCT 481 ACGTGGGCTA CCAGGTGATG CGGGGCCTCA TGCCCCTGGC CTTCTGTGTC CACCCTCTAC 541 TCTACATGGC CGCAGTGCCC AGCCTGGGCT GCTGCTGCCG ACACTGCCCC GGCTACAGGG 601 ACAGCTGGAA CCCAGAGGAC GCCAAGAGCA CTGGCCAAGC CCTGCCCCTC AATGCCACAG 661 CCGCCCCTAA ACCGTCAGAG CCCCAGTCCC GTGAGCTGAG CCAATGCCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA TTTGGCACTT AACCACGTTT AGTCACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt