Construct: ORF TRCN0000466246
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005996.1_s317c1
- Derived from:
- ccsbBroadEn_06252
- DNA Barcode:
- TACACAGCTGCCGTAAATGCAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT2 (2590)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466246
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2590 | GALNT2 | polypeptide N-acetylgalacto... | NM_004481.5 | 99.9% | 100% | 1467G>A |
| 2 | human | 2590 | GALNT2 | polypeptide N-acetylgalacto... | NM_001291866.2 | 92.8% | 92.6% | (many diffs) |
| 3 | human | 2590 | GALNT2 | polypeptide N-acetylgalacto... | XM_017000963.2 | 82% | 78.4% | (many diffs) |
| 4 | human | 2590 | GALNT2 | polypeptide N-acetylgalacto... | XM_017000964.2 | 76.6% | 72.6% | (many diffs) |
| 5 | human | 2590 | GALNT2 | polypeptide N-acetylgalacto... | XM_017000965.1 | 75.9% | 71.9% | (many diffs) |
| 6 | human | 2590 | GALNT2 | polypeptide N-acetylgalacto... | XM_017000966.1 | 71.8% | 68.1% | (many diffs) |
| 7 | mouse | 108148 | Galnt2 | UDP-N-acetyl-alpha-D-galact... | NM_139272.2 | 87.2% | 96.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1779
- ORF length:
- 1713
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gcggcgctcg cggatgctgc tctgcttcgc cttcctgtgg gtgctgggca 121 tcgcctacta catgtactcg gggggcggct ctgcgctggc cgggggcgcg ggcggcggcg 181 ccggcaggaa ggaggactgg aatgaaattg accccattaa aaagaaagac cttcatcaca 241 gcaatggaga agagaaagca caaagcatgg agaccctccc tccagggaaa gtacggtggc 301 cagactttaa ccaggaagct tatgttggag ggacgatggt ccgctccggg caggaccctt 361 acgcccgcaa caagttcaac caggtggaga gtgataagct tcgaatggac agagccatcc 421 ctgacacccg gcatgaccag tgtcagcgga agcagtggcg ggtggatctg ccggccacca 481 gcgtggtgat cacgtttcac aatgaagcca ggtcggccct actcaggacc gtggtcagcg 541 tgcttaagaa aagcccgccc catctcataa aagaaatcat cttggtggat gactacagca 601 atgatcctga ggacggggct ctcttgggga aaattgagaa agtgcgagtt cttagaaatg 661 atcgacgaga aggcctcatg cgctcacggg ttcggggggc cgatgctgcc caagccaagg 721 tcctgacctt cctggacagt cactgcgagt gtaatgagca ctggctggag cccctcctgg 781 aaagggtggc ggaggacagg actcgggttg tgtcacccat catcgatgtc attaatatgg 841 acaactttca gtatgtgggg gcatctgctg acttgaaggg cggttttgat tggaacttgg 901 tattcaagtg ggattacatg acgcctgagc agagaaggtc ccggcagggg aacccagtcg 961 cccctataaa aacccccatg attgctggtg ggctgtttgt gatggataag ttctattttg 1021 aagaactggg gaagtacgac atgatgatgg atgtgtgggg aggagagaac ctagagatct 1081 cgttccgcgt gtggcagtgt ggtggcagcc tggagatcat cccgtgcagc cgtgtgggac 1141 acgtgttccg gaagcagcac ccctacacgt tcccgggtgg cagtggcact gtctttgccc 1201 gaaacacccg ccgggcagca gaggtctgga tggatgaata caaaaatttc tattatgcag 1261 cagtgccttc tgctagaaac gttccttatg gaaatattca gagcagattg gagcttagga 1321 agaaactcag ctgcaagcct ttcaaatggt accttgaaaa tgtctatcca gagttaaggG 1381 TTCCAGACCA TCAGGATATA GCTTTTGGGG CCTTGCAGCA GGGAACTAAC TGCCTCGACA 1441 CTTTGGGACA CTTTGCTGAT GGTGTGGTTG GAGTTTATGA ATGTCACAAT GCTGGGGGAA 1501 ACCAGGAATG GGCCTTGACG AAGGAGAAGT CAGTGAAGCA CATGGATTTG TGCCTTACTG 1561 TGGTGGACCG GGCACCGGGC TCTCTTATAA AGCTGCAGGG CTGCCGAGAA AATGACAGCA 1621 GACAGAAATG GGAACAGATC GAGGGCAACT CCAAGCTGAG GCACGTGGGC AGCAACCTGT 1681 GCCTGGACAG TCGCACGGCC AAGAGCGGGG GCCTAAGCGT GGAGGTGTGT GGCCCGGCCC 1741 TTTCGCAGCA GTGGAAGTTC ACGCTCAACC TGCAGCAGTA CCCAACTTTC TTGTACAAAG 1801 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1861 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1921 ACGATACACA GCTGCCGTAA ATGCAGATAC GCGTTAAGTC gacaatcaac ctctggatta 1981 caaaatttgt gaaagatt