Construct: ORF TRCN0000466259

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015456.1_s317c1
Derived from:
ccsbBroadEn_10557
DNA Barcode:
TGCTCATTAACCGAGATGTATATA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LINC00654 (149837)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466259
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 643406 LOC643406 uncharacterized LOC643406 NR_029405.1 7.6% (many diffs)
2 human 149837 LINC00654 long intergenic non-protein... NR_015406.2 4.7% 1_308del;403A>C;603_6127del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
363
ORF length:
294
Sequence:
1tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG
61TTGGCACCAT GACTGCCTTT GCACTGTCCA TGCTCTCAGC CCACCACCTC CTCCCCCTGC
121CATTGCAGTG GCTAACACTG GAGACGAAGA CCAAGACACC ACCGCCTTTC TCCAGTACCT
181CTCAAATCAG CACAGACAAG GACAAAGGCC TCAATCCACA ACTGCTGAAG ATGGACCCTG
241GCCACATGGG ATGGTCAGAC ACGCCTGCCC AGCTATCTGC AGGCGAAGAG GCTCAGAAGA
301GGTTTAGGGG CCTGAAGGAC ATCTTGCTTC CATGTCCATA TGAGCAGGCT ATTTCTGCTC
361CATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
421TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
481GGCTTTATAT ATCTTGTGGA AAGGACGATG CTCATTAACC GAGATGTATA TAACGCGTTA
541AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)