Construct: ORF TRCN0000466259
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015456.1_s317c1
- Derived from:
- ccsbBroadEn_10557
- DNA Barcode:
- TGCTCATTAACCGAGATGTATATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LINC00654 (149837)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466259
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 643406 | LOC643406 | uncharacterized LOC643406 | NR_029405.1 | 7.6% | (many diffs) | |
| 2 | human | 149837 | LINC00654 | long intergenic non-protein... | NR_015406.2 | 4.7% | 1_308del;403A>C;603_6127del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 363
- ORF length:
- 294
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GACTGCCTTT GCACTGTCCA TGCTCTCAGC CCACCACCTC CTCCCCCTGC 121 CATTGCAGTG GCTAACACTG GAGACGAAGA CCAAGACACC ACCGCCTTTC TCCAGTACCT 181 CTCAAATCAG CACAGACAAG GACAAAGGCC TCAATCCACA ACTGCTGAAG ATGGACCCTG 241 GCCACATGGG ATGGTCAGAC ACGCCTGCCC AGCTATCTGC AGGCGAAGAG GCTCAGAAGA 301 GGTTTAGGGG CCTGAAGGAC ATCTTGCTTC CATGTCCATA TGAGCAGGCT ATTTCTGCTC 361 CATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 421 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 481 GGCTTTATAT ATCTTGTGGA AAGGACGATG CTCATTAACC GAGATGTATA TAACGCGTTA 541 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt