Construct: ORF TRCN0000466261
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005668.1_s317c1
- Derived from:
- ccsbBroadEn_09719
- DNA Barcode:
- CCATGCGCAATTCTGTAATAGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LCN6 (158062)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466261
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 158062 | LCN6 | lipocalin 6 | NM_198946.3 | 99.7% | 100% | 420G>T |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 555
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cggcctgctg ctggctgctt ttctggcttt ggtctcggtg cccagggccc 121 aggccgtgtg gttgggaaga ctggaccctg agcagcttct tgggcccTGG TACGTGCTTG 181 CGGTGGCCTC CCGGGAAAAG GGCTTTGCCA TGGAGAAGGA CATGAAGAAC GTCGTGGGGG 241 TGGTGGTGAC CCTCACTCCA GAAAACAACC TGCGGACGCT GTCCTCTCAG CACGGGCTGG 301 GAGGGTGTGA CCAGAGTGTC ATGGACCTGA TAAAGCGAAA CTCCGGATGG GTGTTTGAGA 361 ATCCCTCAAT AGGCGTGCTG GAGCTCTGGG TGCTGGCCAC CAACTTCAGA GACTATGCCA 421 TCATCTTCAC TCAGCTGGAG TTCGGGGACG AGCCCTTCAA CACCGTGGAG CTGTACAGTC 481 TGACTGAGAC AGCCAGCCAG GAGGCCATGG GGCTCTTCAC CAAGTGGAGC AGGAGCCTGG 541 GCTTCCTGTC ACAGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 601 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 661 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CCATGCGCAA TTCTGTAATA 721 GGCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt