Construct: ORF TRCN0000466276
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007974.1_s317c1
- Derived from:
- ccsbBroadEn_12446
- DNA Barcode:
- TCAATTGTATCAAAAATTAAGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPB41L4A (64097)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466276
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64097 | EPB41L4A | erythrocyte membrane protei... | NM_001347888.2 | 100% | 100% | |
| 2 | human | 64097 | EPB41L4A | erythrocyte membrane protei... | XM_017009689.2 | 28.1% | 28.1% | 556_1974del |
| 3 | human | 64097 | EPB41L4A | erythrocyte membrane protei... | XM_011543533.2 | 27.2% | 27.2% | 556_2040del |
| 4 | human | 64097 | EPB41L4A | erythrocyte membrane protei... | NM_022140.5 | 26.9% | 26.9% | 556_2058del |
| 5 | human | 64097 | EPB41L4A | erythrocyte membrane protei... | XM_011543532.2 | 26.5% | 26.5% | 556_2091del |
| 6 | human | 64097 | EPB41L4A | erythrocyte membrane protei... | XM_011543531.3 | 26.2% | 26.2% | 556_2118del |
| 7 | human | 64097 | EPB41L4A | erythrocyte membrane protei... | NM_001347887.2 | 26.1% | 26.1% | 556_2124del |
| 8 | human | 64097 | EPB41L4A | erythrocyte membrane protei... | XR_001742173.2 | 19.5% | 1_185del;741_2842del | |
| 9 | human | 64097 | EPB41L4A | erythrocyte membrane protei... | NR_144931.2 | 11.7% | 1_238del;794_4715del | |
| 10 | mouse | 13824 | Epb41l4a | erythrocyte membrane protei... | XM_006525628.3 | 26.1% | 27.5% | (many diffs) |
| 11 | mouse | 13824 | Epb41l4a | erythrocyte membrane protei... | NM_013512.2 | 24.6% | 25.9% | (many diffs) |
| 12 | mouse | 13824 | Epb41l4a | erythrocyte membrane protei... | XM_006525627.3 | 20% | 21.1% | (many diffs) |
| 13 | mouse | 13824 | Epb41l4a | erythrocyte membrane protei... | XM_006525629.2 | 16.2% | 16.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 621
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgtttctgc gctgttccgg aagaatttta ctgcgaagtt ttgctcctgg 121 atgaatccaa gttaaccctt accacccagc agcagggcat caagaagtca acgaaaggtt 181 ccgttgtcct tgaccacgta ttccatcacg taaaccttgt ggagatagat tattttgggc 241 TACGTTACTG TGACAGAAGC CATCAGACGT ATTGGCTGGA TCCTGCAAAA ACCCTTGCTG 301 AACACAAAGA ACTGATCAAC ACTGGACCTC CATATACTTT GTATTTTGGT ATTAAATTCT 361 ATGCTGAAGA TCCATGTAAA CTTAAAGAAG AAATAACCAG ATATCAGTTT TTCTTGCAGG 421 TGAAGCAAGA TGTCCTTCAG GGCCGTCTGC CCTGTCCCGT CAACACTGCT GCTCAGCTGG 481 GAGCGTATGC CATCCAGTCG GAGCTTGGAG ATTATGACCC ATATAAACAT ACTGCAGGAT 541 ATGTATCTGA GTACCGGTTT GTTCCTGATC AGAAGGAAGA ACTTGAAGAA GCCATAGAAA 601 GGATTCATAA AACTCTAATG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 661 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 721 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCAA TTGTATCAAA 781 AATTAAGTCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt